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| 产品名称： | TR 310 |
|---|---|
| 商品货号： | TS210746 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Applications： | The antibody reacts with a deteminant on the mouse T cell antigen receptor. |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse T cell receptor |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse T cell receptor |
| Comments： | Animals were immunized with the Tk1 murine T cell lymphoma. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a deteminant on the mouse T cell antigen receptor. It recognizes a determinant encoded by the Vb7 (V beta 7) gene segment. |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640 with 2 mM L-glutamine, 1 mM sodium pyruvate and 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2b |
| Name of Depositor： | IL Weissman |
| Deposited As： | rat (B cell); mouse (myeloma) |