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微信小章| 产品名称： | TIMI.4 |
|---|---|
| 商品货号： | TS210748 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | C57BL/6 |
| Applications： | Derived from a radiation leukemia virus induced thymoma. The line is sensitive to cortisol (0.001 mM) and PHA (0.02 mg/ml). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Derived from a radiation leukemia virus induced thymoma. |
| Antigen Expression： | H-2b; Thy-1.2 |
| Genes Expressed： | H-2b; Thy-1.2 |
| Comments： | Derived from a radiation leukemia virus induced thymoma. The line is sensitive to cortisol (0.001 mM) and PHA (0.02 mg/ml). TL antigen is not expressed. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor： | R Hyman |
| Deposited As： | Mus musculus |
| References： | Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Hyman R. Studies on surface antigen variants. Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 |