微信小陈
微信小章
产品简介
购买须知
| 产品名称： | THB-5 HB-5, HB5 |
|---|---|
| 商品货号： | TS210751 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the human C3d receptor (14500 dalton). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human C3d receptor (CR2, Epstein-Barr virus (EBV) receptor); against CD21 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human C3d receptor (CR2, Epstein-Barr virus (EBV) receptor); against CD21 |
| Comments： | Animals were immunized with the SB human B cell line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the human C3d receptor (14500 dalton). This receptor also is the receptor for Epstein-Barr virus (EBV) on human B cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640, 85%; fetal bovine serum, 15%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | TF Tedder |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Weis JJ, et al. Identification of a 145,000 Mr membrane protein as the C3d receptor (CR2) of human B lymphocytes. Proc. Natl. Acad. Sci. USA 81: 881-885, 1984. PubMed: 6230668 |