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微信小章| 产品名称： | TK-1 |
|---|---|
| 商品货号： | TS210755 |
| Organism： | Mus musculus, mouse |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | AKR/Cum |
| Applications： | TK1 cells have a unique phenotype with high levels of the lymphocyte integrin, alpha 4, beta 7.xa0TK1 cells are useful for studies of alpha 4, beta 7 function and regulation. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | TK1 is a T-cell lymphoma cell line originated by E. C. Butcher and I. L. Weissman from a spontaneous thymic lymphoma/leukemia that developed in an AKR/Cum mouse.
xa0Ref  Butcher EC, et al. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur. J. Immunol. 10: 556-561, 1980. PubMed: 6157544 |
| Antigen Expression： | CD8 +; alpha4, beta7 integrin + |
| Comments： | They bind constitutively to the murine mucosal vascular addressin, MAdCAM-1.xa0
TK1 cells have a unique phenotype with high levels of the lymphocyte integrin, alpha 4, beta 7.xa0 They bind constitutively to the murine mucosal vascular addressin, MAdCAM-1.xa0Ref The cells are CD8 positive; they display approximately 1% of L-selectin of that expressed by normal lymph node cells.xa0 |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM non-essential amino acids, 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 to 2 x 105 viable cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | EC Butcher |
| Deposited As： | mouse |
| References： | Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523 Berlin C, et al. alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell 80: 413-422, 1995. PubMed: 7532110 Butcher EC, et al. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur. J. Immunol. 10: 556-561, 1980. PubMed: 6157544 |