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微信小章| 产品名称： | TFTB1 3916.120.15 |
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| 商品货号： | TS210761 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14). |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the B chain of the plant toxin ricin |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the B chain of the plant toxin ricin |
| Comments： | Animals were immunized with ricin B chain. Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14). The antibody does not cross-react with the ricin A chain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Scrape attached cells. Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Two to three times weekly |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | R Fulton |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Fulton RJ, et al. Purification of ricin A1, A2, and B chains and characterization of their toxicity. J. Biol. Chem. 261: 5314-5319, 1986. PubMed: 3957927 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |