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| 产品名称： | TGP49 |
|---|---|
| 商品货号： | TS210763 |
| Organism： | Mus musculus, transgenic, mouse, transgenic |
| Cell Type： | Epithelial,Epithelial-like |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pancreatic acinar cell tumor; carcinoma |
| Age： | 26 weeks |
| Gender： | male |
| Strain： | Tg(E1a-1-SV40E)Bri18 |
| Applications： | TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse. The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen. In culture, TGP49 cells lost acinar cell morphology and transiently produced somatostatin. The cells do not secrete amylase or lipase. Current stocks of TGP49 are negative for somatostatin and positive for trypsin. |
| Derivation： | TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse. In culture, TGP49 cells lost acinar cell morphology and transiently produced somatostatin. |
| Clinical Data： | TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse. male |
| Comments： | TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse. The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen. In culture, TGP49 cells lost acinar cell morphology and transiently produced somatostatin. Current stocks of TGP49 are negative for somatostatin and positive for trypsin. The cells do not secrete amylase or lipase. Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture. |
| Complete Growth Medium： | A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Twice per week Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | OS Pettengill, D Longnecker |
| Deposited As： | mouse, transgenic |
| References： | Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904 |