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微信小章| 产品名称： | TGP52 |
|---|---|
| 商品货号： | TS210766 |
| Organism： | Mus musculus, transgenic, mouse, transgenic |
| Tissue： | pancreas |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 CELLS CONTAIN PAPOVAVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pancreatic islet cell tumor; insulinoma |
| Age： | 26 weeks |
| Gender： | male |
| Strain： | Tg(E1a-1-SV40E)Bri18 |
| Applications： | TGP52 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an adult male transgenic mouse. The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen. In culture, TGP52 cells transiently produced insulin in culture and then produced somatostatin. Current stocks of TGP52 secrete about 9700 pg/ml of somatostatin in 3 days. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | TGP52 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an adult male transgenic mouse. In culture, TGP52 cells transiently produced insulin in culture and then produced somatostatin. |
| Clinical Data： | TGP52 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an adult male transgenic mouse. male |
| Genes Expressed： | somatostatin |
| Cellular Products： | somatostatin |
| Comments： | TGP52 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an adult male transgenic mouse. The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen. In culture, TGP52 cells transiently produced insulin in culture and then produced somatostatin. Current stocks of TGP52 secrete about 9700 pg/ml of somatostatin in 3 days. Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture. |
| Complete Growth Medium： | A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:4 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | OS Pettengill, D Longnecker |
| Deposited As： | mouse, transgenic |
| References： | Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |