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| 产品名称： | TE4 |
|---|---|
| 商品货号： | TS210775 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with an intracellular antigen expressed in the endocrine thymic epithelium. TE4+ cells strongly express class I and class II histocompatibility antigens, and are positive for thymosin alpha-1 and keratin. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human thymus (thymic endocrine epithelium) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human thymus (thymic endocrine epithelium) |
| Comments： | Animals were immunized with human thymic stroma.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody reacts with an intracellular antigen expressed in the endocrine thymic epithelium.
It defines the mesodermal portion of the thymic microenvironment that may participate in conferring MHC restriction to maturing T lymphocytes.
TE4+ cells strongly express class I and class II histocompatibility antigens, and are positive for thymosin alpha-1 and keratin.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | BF Haynes |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562 |