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微信小章| 产品名称： | TCMK-1 |
|---|---|
| 商品货号： | TS210780 |
| Organism： | Mus musculus, mouse |
| Tissue： | kidney |
| Cell Type： | SV40 transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | weanling |
| Strain： | C3H/Mai |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 78; range = 64 to 157. All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes. |
| Derivation： | The cells are SV40 transformed and are positive for SV40 T-antigen. |
| Antigen Expression： | H-2k |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, in semisolid medium |
| Virus Susceptibility： | Vesicular stomatitis, Glasgow (Indiana)
Human poliovirus 1 |
| Virus Resistance： | poliovirus 1 |
| Comments： | About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | PH Black, W Rowe |
| Deposited As： | Mus musculus |
| Year of Origin： | 1963 |
| References： | Black PH, Rowe WP. SV-40 induced proliferation of tissue culture cells of rabbit, mouse, and porcine origin. Proc. Soc. Exp. Biol. Med. 114: 721-727, 1963. PubMed: 14120333 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |