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| 产品名称： | TBM-54 |
|---|---|
| 商品货号： | TS210787 |
| Organism： | Bufo marinus, toad, tropical |
| Tissue： | urinary bladder |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | TBM-54 is an epithelial cell line derived in 1977 from the urinary bladder of a tropical toad collected in the Dominican Republic. The cells resemble the granular cell type of the urinary bladder. These cells form an epithelium with typical tight junctions and gap junctions and they form multilayers with one layer of undifferentiated cells interposed between the apical layer and the supporting structure. They exhibit hormone sensitive transepithelial electrical resistance, actively transport sodium and are subject to hormonal control in culture. TBM-54 cells do not respond to vasopressin. Aldosterone increases sodium transport in a manner similar to that observed in intact bladder. TBM-54 cells exhibit an excellent response to mineralocortioids in serum free medium. |
| Derivation： | TBM-54 is an epithelial cell line derived in 1977 from the urinary bladder of a tropical toad collected in the Dominican Republic. |
| Comments： | TBM-54 is an epithelial cell line derived in 1977 from the urinary bladder of a tropical toad collected in the Dominican Republic. The cells resemble the granular cell type of the urinary bladder. They have dense granules near the apical surface characteristic of the granular cell type found in the intact bladder. These cells form an epithelium with typical tight junctions and gap junctions and they form multilayers with one layer of undifferentiated cells interposed between the apical layer and the supporting structure. They exhibit hormone sensitive transepithelial electrical resistance, actively transport sodium and are subject to hormonal control in culture. Development of transepithelial electrical resistance (R) and transport rate (Isc) are dependent on time and the density of cells seeded, but steady state Isc and R is characteristic of the cell line and independent of seeding density. TBM-54 cells do not respond to vasopressin. Analogues of cAMP increase sodium transport and urea permeability but do not effect water permeability. Aldosterone increases sodium transport in a manner similar to that observed in intact bladder. TBM-54 cells exhibit an excellent response to mineralocortioids in serum free medium. |
| Complete Growth Medium： | A 7:3 mixture of Coons F-12 and Leibovitzs L-15 medium modified to contain 103 mM NaCl and 25 mM NaHCO3 with 1 nM dexamethasone, 30 nM spermine, 250 nM spermidine, 130 nM putrescine, 0.01 mg/ml insulin, 95%; heat-inactivated fetal bovine serum, 5%
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 3 times per week Remove medium, and rinse the monolayer with fresh 0.25% trypsin solution. Remove the trypsin solution, and let the culture sit at room temperature until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | J Handler, B Rossier |
| Deposited As： | Bufo marinus |
| Year of Origin： | 1977 |
| References： | Handler JS, et al. Toad urinary bladder epithelial cells in culture: maintenance of epithelial structure, sodium transport, and response to hormones. Proc. Natl. Acad. Sci. USA 76: 4151-4155, 1979. PubMed: 226998 Johnson JP, et al. Epithelial organization and hormone sensitivity of toad urinary bladder cells in culture. Am. J. Physiol. 241: F129-F138, 1981. PubMed: 6267945 |