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| 产品名称： | T1 (174 x CEM.T1) |
|---|---|
| 商品货号： | TS210798 |
| Organism： | Homo sapiens, human |
| Tissue： | lymphoblast |
| Cell Type： | B lymphoblast,T lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 xa0Cells contain Epstein-Barr virus viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | T1 is the parental line for the T2 (174 x CEM.T2) line (ATCC CRL-1992), and together the lines are useful for studying antigen processing and T cell recognition of class I major histocompatibility antigens. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line is a cloned hybrid between the 721.174 (variant of the LCL 721 B lymphoblastic cell line) and CEMR.3 (8-azaguanine and ouabain resistant clone of the CEM T lymphoblastic cell line). |
| Antigen Expression： | HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 + |
| Genes Expressed： | HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 + |
| Comments： | The cells express large amounts of HLA DR7 and HLA A2.
It does not express DR3.
|
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X
CSF1PO: 11, 12
D13S317: 11, 12
D16S539:10,12
D5S818: 12, 14
D7S820: 10, 11, 15
THO1: 6, 9.3
TPOX: 8, 12
vWA: 14, 17 |
| Name of Depositor： | P Cresswell |
| Deposited As： | Homo sapiens |
| References： | Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841 |