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| 产品名称： | T27A |
|---|---|
| 商品货号： | TS210800 |
| Organism： | Mus musculus, mouse |
| Tissue： | spleen |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | leukemia |
| Strain： | BALB/c |
| Applications： | This line was derived from a leukemia induced by N-tropic Friend murine leukemia virus. The cells are negative for surface immunoglobulin (sIg-) and Thy-1.2 (Thy-1.2-). Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from a leukemia induced by N-tropic Friend murine leukemia virus. |
| Antigen Expression： | H-2d; Ia8 |
| Genes Expressed： | H-2d; Ia8 |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | This line was derived from a leukemia induced by N-tropic Friend murine leukemia virus. The cells are negative for surface immunoglobulin (sIg-) and Thy-1.2 (Thy-1.2-). Erythroid differentiation and hemoglobin synthesis are NOT inducible by DMSO. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mM 2-mercaptoethanol, 90%; heat-inactivated fetal bovine serum, 10%
|
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. The cells may also be grown as ascites in syngeneic mice. Inject 2 X 10(5) cell per mouse and harvest as soon as abdominal enlargement is observed (about 10 days). Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | B Chesebro |
| Deposited As： | Mus musculus |
| References： | Chesebro B, et al. Lack of erythroid characteristics in Ia-positive leukemia cell lines induced by Friend murine leukemia virus: brief communication. J. Natl. Cancer Inst. 60: 239-242, 1978. PubMed: 564410 Chesebro B, et al. Characterization of Ia8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol. 117: 1267-1274, 1976. PubMed: 185295 |