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微信小章| 产品名称： | T11D7e2 |
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| 商品货号： | TS210802 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with W/Fu rat thymocytes.
Spleen cells were fused with NS-1 myeloma cells.
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| Genes Expressed： | immunoglobulin; monoclonal antibody; against Thy-1.1 antigen; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Thy-1.1 antigen; cytotoxic |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | 50% FBS, 40% complete growth medium, 10% DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2 |
| Isotype： | IgM |
| Name of Depositor： | PA Lake |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Lake P, et al. Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets. Eur. J. Immunol. 9: 875-886, 1979. PubMed: 393521 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |