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微信小章| 产品名称： | T36274 |
|---|---|
| 商品货号： | TS210805 |
| Organism： | Mus musculus, mouse |
| Tissue： | skin |
| Cell Type： | chemically transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | newborn |
| Strain： | BALB/c |
| Applications： | This is one of several lines that are useful in studies of the molecular events in tumor promotion and for development of promotion assays. The T36274 cell line was derived from JB6 Cl 30 by chemical transformation (the cycles of TPA treatment). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The T36274 cell line was derived from JB6 Cl 30 by chemical transformation (the cycles of TPA treatment). |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice and nude mice |
| Comments： | This is one of several lines that are useful in studies of the molecular events in tumor promotion and for development of promotion assays. (see also ATCC CRL-2007 - JB6 Cl 30-7b, ATCC CRL-201 0 - JB6 Cl 41-5a and ATCC CRL-2002 - RT101). The T36274 cell line was derived from JB6 Cl 30 by chemical transformation (the cycles of TPA treatment). |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine, 95%; fetal bovine serum, 5%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: Do not allow the cells to become confluent. The cells must be carried at low density to avoid spontaneous transformation.
Subcultivation Ratio: 1:6 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | NH Colburn |
| Deposited As： | Mus musculus |
| References： | Colburn NH, et al. A cell culture assay for tumor-promoter-dependent progression toward neoplastic phenotype: detection of tumor promoters and promotion inhibitors. Teratog. Carcinog. Mutagen. 1: 87-96, 1980. PubMed: 6119803 Colburn NH, et al. Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. Cancer Res. 38: 624-634, 1978. PubMed: 626967 Colburn NH, et al. Tumour promoter induces anchorage independence irreversibly. Nature 281: 589-591, 1979. PubMed: 492322 Bernstein LR, Colburn NH. AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells. Science 244: 566-569, 1989. PubMed: 2541502 Colburn NH, et al. Dissociation of mitogenesis and late-stage promotion of tumor cell phenotype by phorbol esters: mitogen-resistant variants are sensitive to promotion. Proc. Natl. Acad. Sci. USA 78: 6912-6916, 1981. PubMed: 6947266 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |