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微信小章| 产品名称： | SW948 SW-948 |
|---|---|
| 商品货号： | TS210812 |
| Organism： | Homo sapiens, human |
| Tissue： | colon |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type C, grade III, colorectal adenocarcinoma |
| Age： | 81 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female 81 years Caucasian |
| Antigen Expression： | blood type O; Rh+ |
| Oncogene： | myc +; myb +; ras +; fos +; p53 +; sis -; abl -; ros -; src - |
| Genes Expressed： | carcinoembryonic antigen (CEA) 7 ng/106 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 mL cell sonicate; keratin |
| Cellular Products： | carcinoembryonic antigen (CEA) 7 ng/10 exp6 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 ml cell sonicate; keratin |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.xa0 |
| Comments： | The cells are positive for keratin by immunoperoxidase staining.
The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes.
N-myc and sis oncogene expression were not detected. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37°C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:15 is recommended Medium Renewal: 1 to 2 times per week
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| Culture Conditions： | Atmosphere: air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 10,11 D16S539: 11,12 D5S818: 11 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,11 vWA: 16,18 |
| Isoenzymes： | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor： | A Leibovitz |
| Deposited As： | Homo sapiens |
| References： | Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 |