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微信小章| 产品名称： | SW-13 |
|---|---|
| 商品货号： | TS210824 |
| Organism： | Homo sapiens, human |
| Tissue： | adrenal gland/cortex |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade IV,primary small cell carcinoma |
| Age： | 55 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 63; range = 45 to 65. Approximately 10% of the cells examined possessed a pair of dicentric chromosomes. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Clinical Data： | 55 years Caucasian female |
| Virus Susceptibility： | Human poliovirus 1
Vesicular stomatitis virus |
| Comments： | Electron microscopic studies show many bulb gap junctions (BGJ). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal:xa0Replace the medium every 2 to 3 days.
|
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 9 D16S539: 12 D5S818: 12 D7S820: 8,10 THO1: 7,8 TPOX: 8 vWA: 17,19 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | A Leibovitz |
| Deposited As： | Homo sapiens |
| Year of Origin： | August, 1971 |
| References： | Twenty-Fourth Annual Meeting of the Tissue Culture Association, Boston, MA. In Vitro 8: 443, 1973 Leibovitz A, et al. New human cancer cell culture lines. I. SW-13, small-cell carcinoma of the adrenal cortex. J. Natl. Cancer Inst. 51: 691-697, 1973. PubMed: 4765382 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Lasfargues EY, Ozzello L. Cultivation of human breast carcinomas. J. Natl. Cancer Inst. 21: 1131-1147, 1958. PubMed: 13611537 Johnson RG, Sheridan JD. Junctions between cancer cells in culture: ultrastructure and permeability. Science 174: 717-719, 1971. PubMed: 4330805 Pinto da Silva P, Gilula NB. Gap junctions in normal and transformed fibroblasts in culture. Exp. Cell Res. 71: 393-401, 1972. PubMed: 4339896 |