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微信小章| 产品名称： | SW403 SW-403 |
|---|---|
| 商品货号： | TS210827 |
| Organism： | Homo sapiens, human |
| Tissue： | colon |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type C, grade III, colorectal adenocarcinoma |
| Age： | 51 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 68; range = 59 to 73. The stemline chromosome number is near triploid with 2S component occurring at about 1.4% and 10 marker chromosomes were common to S metaphases. Except for monosomic 1, 13 and 22, and pentasomic 20, most normal chromosome types ranged from disomy to tetrasomy. The cell line is karyotypically very homogeneous and stable. |
| Clinical Data： | 51 years Caucasian female |
| Antigen Expression： | blood type O |
| Genes Expressed： | carcinoembryonic antigen (CEA) 155 ng/106 cells/10 days; keratin,
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| Cellular Products： | carcinoembryonic antigen (CEA) 155 ng/10 exp6 cells/10 days; keratin |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice.
Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments： | CSAp negative (CSAp-).
Colon antigen 3, positive.
The cells are positive for keratin by immunoperoxidase staining. xa0 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100% Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,13 D13S317: 13 D16S539: 10,12 D5S818: 11 D7S820: 8,9 THO1: 6 TPOX: 8,9 vWA: 14,18 |
| Isoenzymes： | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | A Leibovitz |
| Deposited As： | Homo sapiens |
| References： | Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 |