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微信小章| 产品名称： | SW 780 SW-780, SW780 |
|---|---|
| 商品货号： | TS210829 |
| Organism： | Homo sapiens, human |
| Tissue： | urinary bladder |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | transitional cell carcinoma |
| Age： | 80 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | The SW 780 line was established in 1974 by A. Leibovitz from a grade I transitional cell carcinoma. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The SW 780 line was established in 1974 by A. Leibovitz from a grade I transitional cell carcinoma. |
| Clinical Data： | 80 years
Caucasian
female |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | The patient had preoperative chemotherapy (Thiotepa). The cells have a reported plating efficiency of 41%. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:8 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 100% |
| Population Doubling Time： | 38 hrs |
| Name of Depositor： | W McCombs |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1974 |
| References： | Kyriazis AA, et al. Histopathologic evaluation of response to treatment of human tumors grown in the nude mouse. Exp. Cell Biol. 51: 83-95, 1983. PubMed: 6840388 Kyriazis AA, et al. Morphological, biological, and biochemical characteristics of human bladder transitional cell carcinomas grown in tissue culture and in nude mice. Cancer Res. 44: 3997-4005, 1984. PubMed: 6744315 Kyriazis AP, et al. Response to ionizing radiation of human bladder transitional cell carcinomas grown in the nude mouse. Exp. Cell Biol. 53: 281-286, 1985. PubMed: 4043506 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 |