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| 产品名称： | SW 1783 SW-1783, SW1783 |
|---|---|
| 商品货号： | TS210832 |
| Organism： | Homo sapiens, human |
| Tissue： | brain |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade III, astrocytoma |
| Age： | 68 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Karyotype： | hypertetraploid; modal number = 96. The rate of higher ploidies was 10.1%. The markers der(5)t(1;5) (p11;p15), t(1q3q), del(3) (q21), der(6)t(6;11) (p12;p15), der(11)t(6;11) (p12;p15), and 5 others were common to most cells. Of these, der (6) and der(11) had consistently two copies per cell, and were probably formed by balanced translocation between N6 and N11. Nine to 10 marker chromosomes were common to some cells, and 10 to 15 others were detected only once. All normal chromosomes were present in 2 to 5 copies per cell. Generally X had 3 copies and Y had 2 copies per cell. Double minutes (DM) were seen in some cells, and only 1 to a few copies occurred per cell. |
| Derivation： | The SW 1783 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977. The cell line was received at the ATCC in January, 1982 at passage 15. |
| Clinical Data： | 68 years adult Caucasian male |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Culture Conditions： | Atmosphere: air, 100%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,13 D13S317: 9,11 D16S539: 12 D5S818: 12,13 D7S820: 9,13 THO1: 6,8 TPOX: 8,11 vWA: 16,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | A Leibovitz |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1977 |
| References： | Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765 |