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微信小章| 产品名称： | SW 872 SW-872, SW872 |
|---|---|
| 商品货号： | TS210833 |
| Organism： | Homo sapiens, human |
| Tissue： | connective tissue |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | liposarcoma |
| Age： | 36 years adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | hypertriploid; modal number = 80; range = 66 to 81. The rate of higher ploidies was 8.2%.Ten markers were common to most cells. These were: der(5)t(5;?)(q31;?)1, der(5)t(5;?)(q31;?)2, der(6)t(6;?)(q15;?), der(7)t(7;?)(q36;?), t(15q16q) and five others. Both der(5) markers, the der(7) and t(15q16q) were paired.There were 5 copies of N20 and N21, 4 copies of N8, N9, N11, N14 and N17 and a single copy of X in each cell. |
| Derivation： | The SW 872 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a fibrosarcoma removed from a 36 year old male Caucasian. |
| Clinical Data： | Caucasian
male
36 years adult
The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma. |
| Antigen Expression： | Antigen expression: Blood type O+ |
| Tumorigenic： | Yes, |
| Effects： | Yes, produces spindle cell sarcoma in nude mice consistent with liposarcoma |
| Comments： | The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma. An ampule at passage 4 was received at the ATCC in January, 1982. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100%
Temperature: 37°C
|
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | A Leibovitz |
| Deposited As： | Homo sapiens |
| Passage History： | An ampule at passage 4 was received at the ATCC in January, 1982. |
| Year of Origin： | 1974 |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Hu M, et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed: 9270031 |