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微信小章| 产品名称： | SW 684 SW-684, SW684 |
|---|---|
| 商品货号： | TS210837 |
| Organism： | Homo sapiens, human |
| Tissue： | connective tissue |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fibrosarcoma |
| Age： | 68 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | hypertriploid; modal number = 73; range = 59 to 79. The rate of higher ploidies was 9.1%. Eleven markers were common to most cells. These include: der(2)t(2;6)(p13;q13), der(12)t(8;12)(q11;q24), t(15q21q), 19q+, t(8p21q?) and six others. Of these, the der(2) and t(8p21q?) were generally paired. A few cells had double minutes (DM) (one per cell when present). There were 4 copies of N1, N18, N20 and N22 in most cells. Normal 15 and Y were absent. The X was paired in all cells. |
| Derivation： | Temple Texas, United States |
| Clinical Data： | male Caucasian 68 years |
| Tumorigenic： | Yes |
| Effects： | Yes, produces tumors in nude mice consistent with fibrosarcoma. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:5 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 100% |
| Isoenzymes： | AK-1, 1-2 G6PD, B GLO-I, 2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | A Leibovitz |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1974 |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 The SW 684 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a fibrosarcoma removed from a 68 year old male Caucasian. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |