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| 产品名称： | SVG p12 |
|---|---|
| 商品货号： | TS210838 |
| Organism： | Homo sapiens, human |
| Tissue： | brain |
| Cell Type： | astroglia; SV40 transformed |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | fetus, first trimester |
| Applications： | The are susceptible to infection by JC virus, and may be useful in, detecting and cultivating other human neurotropic viruses.
|
| Storage Conditions： | liquid nirogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | The SVG p12 cell line was established by transfecting cultured human fetal glial cells from brain material dissected from 8 to 12 week old embryos with DNA from an ori - mutant of SV40. |
| Genes Expressed： | SV40 T protein; glial fibrillary acidic protein (GFAP) |
| Cellular Products： | SV40 T protein; glial fibrillary acidic protein (GFAP) |
| Virus Susceptibility： | JC polyomavirus
|
| Comments： | The cells express SV40 T antigen.
These cells were recently found to contain infectious BK polyomavirus (BKPyV) of UT strain and a spectrum of defective mutants of this virus.xa0 Ref  Henriksen, S et al. The human fetal glial cell line SVG p12 contains infectious BK polyomavirus (BKPyV). J. Virol. 2014 Apr 23. Epub ahead of print PubMed: 24760884 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： |
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nirogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,11,12 D13S217: 8,13,14 D16S5396: 10,11,12 D5S818: 11,12,14 D7S820: 10,12,13 TH01: 6,7,9.3 TPOX: 8,10,11 vWA: 15,16,18,19 |
| Name of Depositor： | The United States of America |
| U.S. Patent Number： | |
| References： | Major EO. Immortal line of human fetal glial cells. US Patent 4,707,448 dated Nov 17 1987 Henriksen, S et al. The human fetal glial cell line SVG p12 contains infectious BK polyomavirus (BKPyV). J. Virol. 2014 Apr 23. Epub ahead of print PubMed: 24760884 |