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微信小章| 产品名称： | SVR bag4 |
|---|---|
| 商品货号： | TS210839 |
| Organism： | Mus musculus, mouse |
| Tissue： | pancreas |
| Cell Type： | endothelialSV40 transformed |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | SVR bag4 is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280) by infecting SVR cells with a retrovirus encoding beta-galactosidase and puromycin resistance. SVR bag4 can be used as a wild-type control for SVR A221a (ATCC CRL-2386). It can be used to determine if drugs regulate the MAPKK pathway. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | SVR bag4 is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280) by infecting SVR cells with a retrovirus encoding beta-galactosidase and puromycin resistance. |
| Comments： | SVR bag4 is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280) by infecting SVR cells with a retrovirus encoding beta-galactosidase and puromycin resistance. SVR is a hygromycin resistant mouse endothelial cell line containing a temperature sensitive SV40 large T-antigen and H-RAS oncogene. SVR bag4 can be used as a wild-type control for SVR A221a (ATCC CRL-2386). It can be used to determine if drugs regulate the MAPKK pathway. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 1.0 g/L glucose, 95%; fetal bovine serum, 5%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | JL Arbiser |
| Deposited As： | mouse |
| References： | LaMontagne KR Jr., et al. Inhibition of MAP kinase kinase causes morphological reversion and dissociation between soft agar growth and in vivo tumorigenesis in angiosarcoma cells. Am. J. Pathol. 157: 1937-1945, 2000. PubMed: 11106566 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |