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微信小章| 产品名称： | SV-T2 |
|---|---|
| 商品货号： | TS210840 |
| Organism： | Mus musculus, mouse |
| Cell Type： | fibroblastSV40 transformed |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 14 to 17 day gestation embryo |
| Strain： | BALB/c |
| Antigen Expression： | H-2d |
| Genes Expressed： | SV40 T-antigen,H-2d |
| Cellular Products： | SV40 T-antigen |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; bovine calf serum, 10%
|
| Subculturing： | Protocol: Remove medium, add fresh 0.1% trypsin for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Twice per week |
| Name of Depositor： | S Aaronson |
| Deposited As： | Mus musculus |
| References： | Aaronson SA, Todaro GJ. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J. Cell. Physiol. 72: 141-148, 1968. PubMed: 4301006 Aaronson SA, Todaro GJ. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024-1026, 1968. PubMed: 4301647 Petrovic N, et al. Role of proximal promoter elements in regulation of renin gene transcription. J. Biol. Chem. 271: 22499-22505, 1996. PubMed: 8798416 |