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微信小章| 产品名称： | SVR (SVEN 1 ras) |
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| 商品货号： | TS210842 |
| Organism： | Mus musculus, mouse |
| Tissue： | pancreas; islet of Langerhans; endothelium |
| Cell Type： | SV40 transformed |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | C57BL/6 |
| Applications： | The tumors in nude mice cause death by hemorrhage and anemia with thrombocytopenia, and can be used to test angiogenesis inhibitors. |
| Storage Conditions： | liquid nitorgen vapor phase |
| Derivation： | SVR is a derivative of MS1, a pancreatic islet endothelial cell line (see ATCC CRL-2279). |
| Receptor Expression： | vascular endothelial growth factor (VEGF) |
| Genes Expressed： | tissue inhibitor of bioreactive matrix metalloproteinase (high levels) |
| Cellular Products： | tissue inhibitor of bioreactive matrix metalloproteinase (high levels) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms aggressive angiosarcomas in nude mice |
| Comments： | SVR is a derivative of MS1, a pancreatic islet endothelial cell line (see ATCC CRL-2279). MS1 cells were transduced with a retrovirus encoding H-ras and hygromycin resistance. The tumors in nude mice cause death by hemorrhage and anemia with thrombocytopenia, and can be used to test angiogenesis inhibitors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | JL Arbiser |
| Deposited As： | Mus musculus |
| References： | Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347 Tsou R, et al. Retroviral delivery of dominant-negative vascular endothelial growth factor receptor type 2 to murine wounds inhibits wound angiogenesis. Wound Repair Regen. 10: 222-229, 2002. PubMed: 12191004 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |