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微信小章| 产品名称： | SVR A221a |
|---|---|
| 商品货号： | TS210844 |
| Organism： | Mus musculus, mouse |
| Tissue： | pancreas |
| Cell Type： | endothelialSV40 transformed |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | C57BL/6 |
| Applications： | SVR A221a is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280). This cell line along with a wild type control SVR bag4 (ATCC CRL-2287) can be used to determine if drugs regulate the MAPKK pathway. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | SVR A221a is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280). |
| Tumorigenic： | Yes |
| Effects： | Yes, |
| Comments： | SVR A221a is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280). SVR is a hygromycin resistant mouse endothelial cell line containing a temperature sensitive SV40 large T-antigen and H-RAS oncogene. SVR cells were infected with a retrovirus encoding a dominant negative allele of the Mitogen-Activated Protein Kinase Kinase (MAPKK) gene A221a and selected in puromycin. RAS oncogenes upregulate two signal transduction pathways, the MAP Kinase Kinase and phosphoinositol-3-Kinase pathways. Dominant negative MAPKK causes decreased growth in vitro but not in vivo. This cell line along with a wild type control SVR bag4 (ATCC CRL-2287) can be used to determine if drugs regulate the MAPKK pathway. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 1.0 g/L glucose, 95%; fetal bovine serum, 5%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | JL Arbiser |
| Deposited As： | mouse |
| References： | LaMontagne KR Jr., et al. Inhibition of MAP kinase kinase causes morphological reversion and dissociation between soft agar growth and in vivo tumorigenesis in angiosarcoma cells. Am. J. Pathol. 157: 1937-1945, 2000. PubMed: 11106566 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |