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| 产品名称： | SW 1353 SW 1353, SW-1353 |
|---|---|
| 商品货号： | TS210847 |
| Organism： | Homo sapiens, human |
| Tissue： | bone |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | chondrosarcoma |
| Age： | 72 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident |
| Derivation： | The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. |
| Clinical Data： | 72 years Caucasian female The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. |
| Antigen Expression： | Antigen expression: Blood type O- |
| Comments： | The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics. A frozen ampule at passage 12 was received at the ATCC in January, 1982. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 12,13 D16S539: 11,12 D5S818: 10,11 D7S820: 9,11 THO1: 6,9 TPOX: 8,11 vWA: 16,17 |
| Isoenzymes： | AK-1, 1 ES-D, 2 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 2 |
| Name of Depositor： | A Leibovitz |
| Deposited As： | Homo sapiens |
| Passage History： | A frozen ampule at passage 12 was received at the ATCC in January, 1982. |