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微信小章| 产品名称： | SVEC4-10EE2 |
|---|---|
| 商品货号： | TS210851 |
| Organism： | Mus musculus, mouse |
| Tissue： | axillary lymph node; vascular epithelium |
| Cell Type： | endothelial, SV40 transformed |
| Product Format： | frozen |
| Morphology： | cobblestone |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | male |
| Strain： | C3H/HeJ |
| Applications： | SVEC4-10EE2 is an endothelial cell line derived from the same solid tumor as SVEC4-10EHR1 (see CRL-2161) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181). The cells were cloned in 1989 by limiting dilution, and are distinguished by their inability to differentiate on a synthetic basement-like membrane. The cells exhibit a stable cobblestone morphology in contrast to its sibling from the same tumor (SVEC4-10EHR1). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | SVEC4-10EE2 is an endothelial cell line derived from the same solid tumor as SVEC4-10EHR1 (see CRL-2161) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181). |
| Clinical Data： | male |
| Antigen Expression： | H-2 K; Factor VIII related antigen; VCAM |
| Receptor Expression： | high affinity receptors for low density lipoprotein (LDL) |
| Genes Expressed： | H-2 K; Factor VIII related antigen; VCAM |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells induce spindle tumors with some of the histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks |
| Comments： | SVEC4-10EE2 is an endothelial cell line derived from the same solid tumor as SVEC4-10EHR1 (see CRL-2161) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181). The cells were cloned in 1989 by limiting dilution, and are distinguished by their inability to differentiate on a synthetic basement-like membrane. They form tube-like structures in the presence of suramin, a drug which blocks growth factor receptors. The cells exhibit a stable cobblestone morphology in contrast to its sibling from the same tumor (SVEC4-10EHR1). The cells express the cell surface major histocompatibility complex class I antigen, H-2 k of the parental cell line, and express Factor VIII related antigen, high affinity receptors for low density lipoprotein and vascular cell adhesion molecule (VCAM). The cells stain positively for SV40 T antigen. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | KA OConnell |
| Year of Origin： | 1989 |
| References： | OConnell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 OConnell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposis sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 OConnell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposis sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |