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微信小章| 产品名称： | SV40LT-SMC Clone HEP-SA |
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| 商品货号： | TS210853 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | aorta; smooth muscle |
| Cell Type： | SV40 transfected |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 3 months |
| Strain： | Sprague-Dawley |
| Applications： | This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen. |
| Genes Expressed： | alpha actin; large T antigen |
| Cellular Products： | alpha actin; large T antigen |
| Comments： | This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen. Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance). Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin. Large T antigen is detectable in the nuclei. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 10% Bovine Calf Serum and 200 mcg/mL G418
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | CF Reilly, R McFall |
| Deposited As： | Rattus sp. |
| References： | Reilly CF. Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin. J. Cell. Physiol. 142: 342-351, 1990. PubMed: 2154504 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |