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微信小章| 产品名称： | SV40 MES 13 |
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| 商品货号： | TS210854 |
| Organism： | Mus musculus, mouse |
| Tissue： | kidney/glomerulus |
| Cell Type： | mesangial cell |
| Product Format： | frozen |
| Morphology： | myofibroblast-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 7 to 10 weeks |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was established from the kidney of a mouse transgenic for the SV40 early region. |
| Effects： | Yes, forms colonies in soft agar |
| Comments： | The cells form colonies in soft agar, and are positive for SV40 large T antigen. The cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm.xa0 They are reported to contract in the presence of 1 X 10-6 M angiotensin II. The cells form colonies in soft agar, and are positive for SV40 large T antigen. |
| Complete Growth Medium： | The base medium for this cell line is 3:1 mixture of ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002 and Hams F12 medium with 14 mM HEPES.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Time： | 26 hrs |
| Name of Depositor： | LJ Striker |
| Deposited As： | Mus musculus |
| References： | MacKay K, et al. Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice. Kidney Int. 33: 677-684, 1988. PubMed: 2835539 Robey RB, et al. Regulation of mesangial cell hexokinase activity by PKC and the classic MAPK pathway. Am. J. Physiol. 277: F742-F749, 1999. PubMed: 10564237 Robey RB, et al. Thrombin is a novel regulator of hexokinase activity in mesangial cells. Kidney Int. 57: 2308-2318, 2000. PubMed: 10844601 Robey RB, et al. Regulation of mesangial cell hexokinase activity and expression by heparin-binding epidermal growth factor-like growth factor: epidermal growth factors and phorbol esters increase glucose metabolism via a common mechanism involving classic mitogen-activated protein kinase pathway activation and induction of hexokinase II expression. J. Biol. Chem. 277: 14370-14378, 2002. PubMed: 11782486 Maile S, et al. The morphology of mesangial cells cultured at high density and in collagen gels. Histol. Histopathol. 15: 403-414, 2000. PubMed: 10809358 Coy PE, et al. LPA is a novel lipid regulator of mesangial cell hexokinase activity and HKII isoform expression. Am. J. Physiol. Renal Physiol. 283: F271-F279, 2002. PubMed: 12110510 This line was established from the kidney of a mouse transgenic for the SV40 early region. Taneja N, et al. Proinflammatory interleukin-1 cytokines increase mesangial cell hexokinase activity and hexokinase II isoform abundance. Am. J. Physiol. Cell Physiol. 287: C548-C557, 2004. PubMed: 15070811 |