微信小陈
微信小章| 产品名称： | SUP-B15 |
|---|---|
| 商品货号： | TS210855 |
| Organism： | Homo sapiens, human |
| Tissue： | bone marrow |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute lymphoblastic leukemia |
| Age： | 8 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | 46, XY; the following markers are present: t(9;22)(q34;q11), t(4;14) (p11;q24), der(4)t(1;4) (p11;q33), t(9;22) (q34;q11), der(10)t(3;10) (q25;q26), add(16); Philadelphia chromosome is present. |
| Images： |  |
| Derivation： | This line was was derived from malignant cells collected from the bone marrow of an 8 year old child with Philadelphia chromosome positive B cell ALL. |
| Clinical Data： | 8 years Caucasian male xad |
| Antigen Expression： | CD1a -; CD2 -; CD3 -; CD4 -; CD5 -; CD8 -; CD10 +; CD13 +; CD38 +; CD71 +; HLA DR + |
| Genes Expressed： | immunoglobulin (cytoplasmic) |
| Cellular Products： | immunoglobulin (cytoplasmic) |
| Comments： | The cells express multiple B lineage markers, but do not express T cell markers.
The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens. They are are negative for CB1, Leu 1 (CD5), Leu2 (CD8), Leu3 (CD4), Leu4 (CD3), Leu5 (CD2), Leu6 (CD1a), Leu9, Leu M1 (CD15), My9 (CD33), surface immunoglobulin (sIg -) and Epstein-Barr virus.
|
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 105 viable cells/mL and maintain between 5 X 105 and 2 X 106 cells/mL.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,14 D16S539: 11,12 D5S818: 12,13 D7S820: 10,11 THO1: 6,9.3 TPOX: 8,9 vWA: 15,17 |
| Population Doubling Time： | 18 hrs |
| Name of Depositor： | SD Smith |
| Deposited As： | Homo sapiens |
| References： | Fainstein E, et al. A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia. Nature 330: 386-389, 1987. PubMed: 2825022 Clark SS, et al. Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL). Science 239: 775-777, 1988. PubMed: 3422516 Naumovski L, et al. Philadelphia chromosome-positive acute lymphoblastic leukemia cell lines without classical breakpoint cluster region rearrangement. Cancer Res. 48: 2876-2879, 1988. PubMed: 3162827 Rubin CM, et al. Heterogeneity of genomic fusion of BCR and ABL in Philadelphia chromosome-positive acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 85: 2795-2799, 1988. PubMed: 2833755 Kawasaki ES, et al. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc. Natl. Acad. Sci. USA 85: 5698-5702, 1988. PubMed: 3165197 |