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微信小章| 产品名称： | Super Dome |
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| 商品货号： | TS210857 |
| Organism： | Canis familiaris, dog |
| Tissue： | kidney |
| Cell Type： | Epithelial,Epithelial-like |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Gender： | female |
| Applications： | Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. |
| Clinical Data： | Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. female |
| Comments： | Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. Super Dome forms domes that are approximately 5 times the area of MDCK. For dome formation, the cells should be fed twice a day by a complete medium change. |
| Complete Growth Medium： | These cells are grown in a medium containing a 1:1 mixture of Dulbeccos Modified Eagles Medium and Hams F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10% fetal bovine serum.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | RJ Klebe |
| References： | Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |