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购买须知
| 产品名称： | ST |
|---|---|
| 商品货号： | TS210865 |
| Organism： | Sus scrofa, pig |
| Tissue： | testis |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | 80 to 90 days gestation |
| Gender： | male |
| Applications： | This line is useful for the propagation, assay and isolation of PPV, TGE and swine enteroviruses. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | diploid |
| Images： |  |
| Clinical Data： | male
80 to 90 days gestation |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | SJ Wessman |
| Deposited As： | Sus scrofa |
| References： | McClurkin AW, Norman JO. Studies on transmissible gastroenteritis of swine. II. Selected characteristics of a cytopathogenic virus common to five isolates from transmissible gastroenteritis. Can. J. Comp. Med. Vet. Sci. 30: 190-198, 1966. PubMed: 4224292 |