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| 产品名称： | SR |
|---|---|
| 商品货号： | TS210867 |
| Organism： | Homo sapiens, human |
| Tissue： | pleural effusion |
| Cell Type： | lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | large cell immunoblastic lymphoma |
| Age： | 11 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo. |
| Clinical Data： | 11 years Caucasian male |
| Antigen Expression： | Hle-1 +; HLA DQ +; HLA DR +; CD25 +; CD19 -; CD20 -; CD21 -; CD22 -; T cell receptor (TCR) - |
| Comments： | SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages. Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition. SR cells have been reported to be Epstein-Barr virus genome negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 -xa02 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal: 2 to 3 times a week. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 14,15 D13S317: 11 D16S539: 11,13 D5S818: 11 D7S820: 9,12 THO1: 6,7 TPOX: 8,11 vWA: 17,18 |
| Name of Depositor： | M Beckwith |
| Deposited As： | Homo sapiens |
| References： | Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723 |