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| 产品名称： | SNU-C2A |
|---|---|
| 商品货号： | TS210869 |
| Organism： | Homo sapiens, human |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | loosely adherent, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | colorectal carcinoma |
| Age： | 43 years |
| Gender： | female |
| Ethnicity： | Asian |
| Applications： | This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985. The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 46; range = 44 to 48. This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 86%, with polyploidy at 16.2%. The ins(1;?)(p13;?), del(3)(p23), t(4q,?), t(8;?), del(9)(p13), del(11)(p13), 14q+ and two other marker chromosomes are common to all metaphases examined. Other altered chromosomes, if any, occur only sporadically. Normal N11 is absent. Two copies of X chromosomes are found in each cell. The Y chromosome is not found in QM stained preparations. |
| Images： |  |
| Derivation： | This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985. The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy. |
| Clinical Data： | The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy. female Asian 43 years |
| Antigen Expression： | Blood type A; Rh+ |
| Genes Expressed： | carcinoembryonic antigen (CEA), 5 ng/ml per 10 exp6 cells per 10 days; TAG-72 surface antigen,Blood type A; Rh+ |
| Cellular Products： | carcinoembryonic antigen (CEA), 5 ng/ml per 10 exp6 cells per 10 days; TAG-72 surface antigen |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985. The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy. The line was initially grown in RPMI 1640 medium with 20% fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4 (it can also be grown in medium with 10% serum). The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen. |
| Complete Growth Medium： | ACL-4 medium supplemented with 10% FBS The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Protocol: Cultures can be maintained by scraping off the attached cells and transfer along with the the floating cells into new flasks.
Medium Renewal: Two times weekly
Note: Growth is slow. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additonal 10% fetal bovine serum and 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X
CSF1PO: 11, 14
D13S317: 9, 12
D16S539: 8, 9
D5S818: 8, 9
D7S820: 13, 16
THO1: 9
TPOX: 8
vWA: 15, 19, 20
Species confirmation via CO1 assay: Human (mouse material may also be detected, likely due to this cell line being a nude mouse xenograft of tumor fragments from a human patient) |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | AF Gazdar |
| Deposited As： | Homo sapiens |
| Passage History： | The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy. |
| Year of Origin： | 1982 |
| References： | Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249 Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells |