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微信小章| 产品名称： | SOB-15 |
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| 商品货号： | TS210870 |
| Organism： | Oncorhynchus mykiss, trout, rainbow |
| Tissue： | liver, epithelial |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | spindle-like with polygonal foci |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Shasta |
| Storage Conditions： | liquid nitrogen vapor phase |
| Comments： | The cells retain characteristics of normal fish liver |
| Complete Growth Medium： | Minium essential Medium Eagle with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate and supplemented with 2 mM L-glutamine, 1.32 g/L sodium chloride, 0.1 mM non-essential amino acids and 10% heat-inactivated fetal bovine serum.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 17°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | GK Ostrander, JB Blair |
| References： | Ostrander GK, et al. Long-term primary culture epithelia cells from rainbow trout (Oncorhynchus mykiss) liver. In Vitro Cell. Dev. Biol. Anim. 31: 367-378, 1995. PubMed: 7543343 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Blair JB, et al. Isolated trout liver cells: establishing short-term primary cultures exhibiting cell-to-cell interactions. In Vitro Cell Dev Biol 26(3 Pt 1):237-49, 1990. PubMed: 2318789 |