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| 产品名称： | SNU-475 |
|---|---|
| 商品货号： | TS210871 |
| Organism： | Homo sapiens, human |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent, monolayer of diffusely spreading cells |
| Biosafety Level： | 2 xa0Cells contain Hepatitis B virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade II-IV/V, hepatocellular carcinoma |
| Age： | 43 years |
| Gender： | male |
| Ethnicity： | Asian |
| Karyotype： | aneuploid; modal number = 61 |
| Derivation： | SNU-475 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy.
Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. |
| Clinical Data： | 43 years
Asian
male
|
| Antigen Expression： | Blood Type AB; Rh + |
| Comments： | Grossly, the original tumor was massive growth type. Histologically, it was predominantly compact and minor trabecular type.
The cultured cells are multinucleated. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.
HBV genomic RNA was not expressed. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:6 Medium Renewal: 2 to 3 timesa a week. xa0
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,11 D16S539: 12 D5S818: 10,13 D7S820: 7,12 THO1: 7,9 TPOX: 8,9 vWA: 14 |
| Population Doubling Time： | 66 hrs |
| Name of Depositor： | J Park |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1990 |
| References： | Park JG, et al. Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080 |