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| 产品名称： | SNU-C2B |
|---|---|
| 商品货号： | TS210873 |
| Organism： | Homo sapiens, human |
| Tissue： | cecum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | loosely adherent, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | colorectal carcinoma |
| Age： | 43 years |
| Gender： | female |
| Ethnicity： | Asian |
| Applications： | This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985. The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also TS210873.1 which was derived from the third nude mouse passage). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 46; range = 45 to 48. This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 70% with polyploidy at 16.4%. The del (3) (p23), t(4,?), t(8;?) (p22), del (9) (p13), del (11) (p13), t(1;8) (p13;p24), t(1;?) (q11;?) and 2 other marker chromosomes are common to all metaphases examined. Other structurally altered chromosomes, if any, occur only sporadically. The t(1;8) and t(1;?) are not found in CCL-250.1. Normal N8 and N11 are absent. The X chromosomes are two copies per cell. The Y chromosome is not detected by Q-banding. |
| Derivation： | This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985. The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also TS210873.1 which was derived from the third nude mouse passage). |
| Clinical Data： | female
Asian
43 years |
| Antigen Expression： | Blood type A; Rh+ |
| Receptor Expression： | epidermal growth factor (EGF) |
| Genes Expressed： | carcinoembryonic antigen (CEA), 24 ng/ml per 10 exp6 cells per 10 days,Blood type A; Rh+ |
| Cellular Products： | carcinoembryonic antigen (CEA), 24 ng/ml per 10 exp6 cells per 10 days |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | The cells have a high concentration of receptors for EGF. Note that this line has lost both chromosomes 8 and 11 while SNU-C2A (TS210873.1) has lost only chromosome 11. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： |
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X
CSF1PO: 11, 14
D13S317: 9, 10, 11
D16S539: 8, 9
D5S818: 8, 9
D7S820: 13, 16
THO1: 9
TPOX: 8
vWA: 15, 18, 19
Species confirmation via CO1 assay: Human (mouse material may also be detected, likely due to this cell line being a nude mouse xenograft of tumor fragments from a human patient) |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | AF Gazdar |
| Deposited As： | Homo sapiens |
| Passage History： | The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also TS210873.1 which was derived from the third nude mouse passage). |
| Year of Origin： | 1982 |
| References： | Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249 |