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微信小章| 产品名称： | Sp2/0-Ag14 |
|---|---|
| 商品货号： | TS210876 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | Spleen |
| Cell Type： | Hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | Lymphoblast-like |
| Culture Properties： | Suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c |
| Applications： | This cell line is a fusion partner for production of somatic cell hybrids. |
| Storage Conditions： | Liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. |
| Antigen Expression： | Antigen expression: H-2d |
| Comments： | The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 μg/ml and are HAT sensitive.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Initiate the cells at a start-up seeding density of 1.0 x 10e5 viable cells/mL. Maintain cell density between 1 X 10e4 and 1 X 10e6 viable cells/mL. Some cells can attach and be transferred by shaking them loose.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | G Kohler |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293 Shulman M, et al. A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-270, 1978. PubMed: 714156 Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022 |
| Cross References： | Nucleotide (GenBank) : NC_001702 Murine type C retrovirus, complete genome. Nucleotide (GenBank) : X94150 Retroviridae complete genome (murine type C retrovirus). |