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微信小章| 产品名称： | Sol8 |
|---|---|
| 商品货号： | TS210877 |
| Organism： | Mus musculus, mouse |
| Tissue： | skeletal muscle |
| Cell Type： | myoblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 4 weeks |
| Strain： | C3H |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Sol8 is a myogenic cell line isolated by Daubas et al. from primary cultures of soleus muscle taken from the leg of a normal C3H mouse. The cells were split two times and then cloned by limiting dilution. |
| Comments： | Sol8 cells will differentiate into myotubes with serum deprivation. They have a phenotype similar to slow twitch fibers. Note: Do not allow the cells to become confluent. The myoblast component of the population will rapidly become depleted if the culture becomes confluent. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: Never allow the cells to become confluent. Subculture at 50% of confluence.
Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:12 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Population Doubling Time： | 16 to 18 hrs |
| Name of Depositor： | R Bassel-Duby |
| Deposited As： | Mus musculus |
| References： | Daubas P, et al. Functional activity of the two promoters of the myosin alkali light chain gene in primary muscle cell cultures: comparison with other muscle gene promoters and other culture systems. Nucleic Acids Res. 16: 1251-1271, 1988. PubMed: 2894633 Li CY, et al. Potential role of WAF1/Cip1/p21 as a mediator of TGF-beta cytoinhibitory effect. J. Biol. Chem. 270: 4971-4974, 1995. PubMed: 7890601 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |