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| 产品名称： | SL-29 |
|---|---|
| 商品货号： | TS210881 |
| Organism： | Gallus gallus, chicken |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo; 11 days gestation |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | This cell line was developed by standard trypsinization of a decapitated 11-day SPAFAS leghorn embryo. |
| Comments： | The cells have a doubling potential of 35 population doublings. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: tryptose phosphate broth to a final concentration of 5% and fetal bovine serum to a final concentration of 5%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: An inoculation density of 5 X 104 viable cells per cm2 of flask or dish surface area is recommended
Medium Renewal: 2 to 3 times per week
Note: The tryptose phosphate broth should be prepared fresh every 2 weeks. Fresh glutamine is also very important for the growth of this line. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Capacity： | The cells have a doubling potential of 35 population doublings. |
| Name of Depositor： | RJ Hay |
| Deposited As： | Gallus gallus |
| References： | . Aging in cell and tissue cultures. New York: Plenum Press; 1970. Ramirez AD, et al. Antisporozoite antibodies with protective and nonprotective activities: in vitro and in vivo correlations using Plasmodium gallinaceum, an avian model. J. Eukaryot. Microbiol. 42: 705-708, 1995. PubMed: 8520586 |