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| 产品名称： | SNU-16 |
|---|---|
| 商品货号： | TS210883 |
| Organism： | Homo sapiens, human |
| Tissue： | stomach; derived from metastatic site: ascites |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | gastric carcinoma |
| Age： | 33 years |
| Gender： | female |
| Ethnicity： | Asian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found. |
| Derivation： | SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy.xa0 |
| Clinical Data： | 33 years Asian female SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. |
| Antigen Expression： | Blood Type A; Rh + The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72. |
| Receptor Expression： | vasoactive intestinal peptide (VIP), expressed Ref   Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397 |
| Oncogene： | myc +; erb B2 + |
| Genes Expressed： | myc +; erb B2 +,Blood Type A; Rh +,The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72. |
| Effects： | Yes, the cells have a reported colony forming efficiency of 10% in semisolid medium. |
| Comments： | SNU-16 cells were positive for VIP receptors but lacked gastrin receptors.
The line was established from cells taken prior to chemotherapy.
No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.
The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 8,12 D16S539: 11,13 D5S818: 10,13 D7S820: 12 THO1: 6,9 TPOX: 11 vWA: 16 |
| Population Doubling Time： | 27 hrs |
| Name of Depositor： | J Park |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1987 |
| References： | Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |