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微信小章| 产品名称： | SK-N-SH |
|---|---|
| 商品货号： | TS210890 |
| Organism： | Homo sapiens, human |
| Tissue： | brain; derived from metastatic site: bone marrow |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Age： | 4 years |
| Gender： | female |
| Applications： | SK-N-SH has been used as a target cell line in cell mediated cytotoxicity assays and is a suitable transfection host. |
| Karyotype： | The cell line is hyperdiploid human female (XX), with the modal chromosome number of 47. Normal chromosomes N9 and N22 are single. One copy of each of these chromosomes is structurally altered to form the two marker chromosomes 9q+ and 22q+., Chromosomes N7 is trisomic. Extra bands were found on one copy of chromosome N7, thereby forming a marker chromosome as described by R.C. Seeger. May have been translocated in part(s) to the q arms of chromosomes N9 and N22. |
| Images： |  |
| Derivation： | The SK-N-SH line was developed by J.L. Biedler and differs from SK-N-MC (see ATCC HTB-10) in that it exhibits a longer doubling time and higher levels of dopamine - beta - hydroxylase. |
| Clinical Data： | female |
| Antigen Expression： | Blood Type A; Rh+ |
| Genes Expressed： | plasminogen activator,shows increased expression of M-CSF after treatment with amyloid-beta peptide. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53% EDTA solution (ATCC® 30-2101™). Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 8,13 D5S818: 12 D7S820: 7,10 THO1: 7,10 TPOX: 8,11 vWA: 14,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | G Trempe, LJ Old |
| Deposited As： | Homo sapiens |
| References： | Gilbert LC, Wachsman JT. Characterization and partial purification of the plasminogen activator from human neuroblastoma cell line, SK-N-SH. A comparison with human urokinase. Biochim. Biophys. Acta 704: 450-460, 1982. PubMed: 7052133 Spengler BA, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells established in vitro. In Vitro 8: 410, 1973. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013 Seeger RC, et al. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37: 1364-1371, 1977. PubMed: 856461 Yan SD, et al. Amyloid-beta peptide-Receptor for Advanced Glycation Endproduct interaction elicits neuronal expression of macrophage-colony stimulating factor: A proinflammatory pathway in Alzheimer disease. Proc. Natl. Acad. Sci. USA 94: 5296-5301, 1997. PubMed: 9144231 Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 Chang YE, et al. Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. PubMed: 8648731 He B, et al. The carboxyl terminus of the murine MyD116 gene substitutes for the corresponding domain of the gamma134.5 gene of herpes simplex virus to preclude the premature shutoff of total protein synthesis in infected human cells. J. Virol. 70: 84-90, 1996. PubMed: 8523596 Yoshikawa T, et al. Downstream regulatory elements increase acute and latent herpes simplex virus type 2 latency-associated transcript expression but do not influence recurrence phenotype or establishment of latency. J. Virol. 70: 1535-1541, 1996. PubMed: 8627672 |