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微信小章| 产品名称： | SKW 6.4 |
|---|---|
| 商品货号： | TS210892 |
| Organism： | Homo sapiens, human |
| Cell Type： | B lymphocyte; Epstein-Barr virus (EBV) transformed |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | immunoglobulin; monoclonal antibody |
| Cellular Products： | immunoglobulin; monoclonal antibody |
| Comments： | The cells respond to T Cell Replacing Factor (TRF) and B Cell Differentiation Factor (BCDF) by secreting IgM. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 4 x 105 and 2 x 106xa0cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-Xxa0 |
| Culture Conditions： | Temperature: 37°C
Atmosphere: 5% CO2 |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 THO1: 8,9.3 TPOX: 8,11 vWA: 17,20 |
| Isotype： | IgM |
| Name of Depositor： | P Ralph |
| Deposited As： | Homo sapiens |
| References： | Saiki O, Ralph P. Clonal differences in response to T cell replacing factor (TRF) for IgM secretion and TRF receptors in a human B lymphoblast cell line. Eur. J. Immunol. 13: 31-34, 1983. PubMed: 6339249 Ralph P, et al. IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester. Immunol. Lett. 7: 17-23, 1983. PubMed: 6605913 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |