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微信小章| 产品名称： | SK-PN-DW |
|---|---|
| 商品货号： | TS210894 |
| Organism： | Homo sapiens, human |
| Tissue： | retroperitoneal embryonal tumor |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | malignant primitive neuroectodermal tumor |
| Age： | 17 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | aneuploid; modal number = 40 to 41; monosomy 13 was found in 100% of the cells; iso(17q) and monosomy 9, 10, 11, and 18 were found in greater than 20% of cells analyzed., In a large proportion of the cells, the double minutes per cell were too numerous to count. |
| Derivation： | SK-PN-DW is a primitive neuroectodermal tumor (PNET) cell line derived in 1979 from a 17 year old Caucasian male with embryonal retroperitoneal tumor. |
| Clinical Data： | 17 years
male
Caucasian |
| Genes Expressed： | Cholecystokinin (CCK) |
| Cellular Products： | Cholecystokinin (CCK) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | The cells express CCK specific mRNA and synthesize considerable quantities of variably processed CCK prohormone. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 inCulture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 9 D16S539: 12 D5S818: 13,14 D7S820: 8,14 THO1: 6 TPOX: 8,9 vWA: 16 |
| Name of Depositor： | C Helson |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1979 |
| References： | Potluri VR, et al. Primitive neuroectodermal tumor cell lines: chromosomal analysis of five cases. Cancer Genet. Cytogenet. 24: 75-86, 1987. PubMed: 3024811 Schneider BS, et al. Expression of the cholecystokinin gene by cultured human primitive neuroepithelioma cell lines. J. Clin. Endocrinol. Metab. 69: 411-419, 1989. PubMed: 2753982 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |