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微信小章| 产品名称： | SK-N-AS |
|---|---|
| 商品货号： | TS210898 |
| Organism： | Homo sapiens, human |
| Tissue： | brain; derived from metastatic site: bone marrow |
| Cell Type： | neuroblast |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Age： | 6 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma. |
| Clinical Data： | 6 years Caucasian female |
| Receptor Expression： | insulin-like growth factor I (IGF-I), expressed |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | SK-N-AS cells synthesize Insulin-like growth factor II (IFG-II) and IGF-II RNA and possess type I IGF receptors. Ref   El-Badry OM, et al. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840 Retinoic acid partially inhibits proliferation, and cells fail to differentiate. RefGaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405 xa0The cells exhibit low or no MDR1 expression. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:5 to 1:10 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Freeze Medium: Culture medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 9 D16S539: 14 D5S818: 11,12 D7S820: 11,13 THO1: 9.3 TPOX: 11,12 vWA: 16,17 |
| Population Doubling Time： | 39 hours |
| Name of Depositor： | C Helson |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1981 |
| References： | Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792 Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824 Gaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405 El-Badry OM, et al. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840 bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma |