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微信小章| 产品名称： | SK-N-BE(2) |
|---|---|
| 商品货号： | TS210899 |
| Organism： | Homo sapiens, human |
| Tissue： | brain; derived from metastatic site: bone marrow |
| Cell Type： | neuroblast, Neuroblastoma |
| Product Format： | frozen |
| Morphology： | neuroblast |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Age： | 2 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 44; one or more HSR bearing chromosomes |
| Images： |  |
| Derivation： | The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. |
| Clinical Data： | male
2 years |
| Tumorigenic： | Yes |
| Effects： | Yes, an inoculum of 107 cells produced tumors in cortisonized hamster cheek pouches with 18% frequency |
| Comments： | The cells exhibit moderate levels of dopamine beta hydroxylase activity.
SK-N-BE(2) cells have a reported saturation density greater than 1 X 106 cells/cm2.
The morphology of the cells varies with some cells having long processes and others that are epithelioid like.
The cells will aggregate, form clumps and float. |
| Complete Growth Medium： | The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | These cells grow as a mixture of floating and adherent cells. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with a fresh 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 mL of trypsin solution, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:12 to 1:20 is recommended.
Medium Renewal: Everyxa04 toxa07 days. |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10 D13S317: 11 D16S539: 9,11 D5S818: 12 D7S820: 9,10 THO1: 6,7 TPOX: 8,11 vWA: 18 |
| Population Doubling Time： | 30 hrs |
| Name of Depositor： | JL Biedler |
| Deposited As： | Homo sapiens |
| Year of Origin： | November, 1972 |
| References： | Biedler JL, Spengler BA. A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture. J. Natl. Cancer Inst. 57: 683-695, 1976. PubMed: 62055 Barnes EN, et al. The fine structure of continuous human neuroblastoma lines SK-N-SH, SK- N-BE(2), and SK-N-MC. In Vitro 17: 619-131, 1981. PubMed: 7327593 Biedler JL, Spengler BA. Metaphase chromosome anomaly: association with drug resistance and cell-specific products. Science 191: 185-187, 1976. PubMed: 942798 Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704 |