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微信小章| 产品名称： | SK-MEL-31 |
|---|---|
| 商品货号： | TS210900 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | epithelial cell |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Malignant melanoma |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 82; range = 74 to 84. This is a hypotetra-/hypertriploid human cell line. The modal chromosome number is 82 occurring at 16% with polyploidy at 10.3%. The markers del(1)(q23), t(1q10p), del(7)(p13), t(7p18q), i(21q) and 5 others are common to all cells. They are all paired in most cells. N1 and N18 are absent, N11 is single-copied, and N6, N7, N8 and N16 are generally 4-copied (many cells also had 5 copies each for N7 and N8). The X is paired. |
| Derivation： | This is one of a very extensive series of melanoma lines (see also ATCC HTB-70, ATCC HTB-71 and ATCC HTB-72) isolated by T. Takahashi and associates. |
| Clinical Data： | female |
| Antigen Expression： | Antigen expression: HLA A3,A29, Bw35,B40, Cw3,Cw4, DRw4 |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; produces malignant melanoma |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 85%; fetal bovine serum, 15%
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| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 13 D5S818: 11,13 D7S820: 10 THO1: 6,9 TPOX: 8,11 vWA: 16,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | T Takahashi |
| Deposited As： | Homo sapiens |
| References： | Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619 Guldberg P, et al. Disruption of the MMAC1/PTEN gene by deletion or mutation is a frequent event in malignant melanoma. Cancer Res. 57: 3660-3663, 1997. PubMed: 9288767 |