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微信小章| 产品名称： | SK-N-FI |
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| 商品货号： | TS210907 |
| Organism： | Homo sapiens, human |
| Tissue： | brain; derived from metastatic site: bone marrow |
| Cell Type： | neuroblast |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Age： | 11 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | SK-N-FI is a neuroblastoma cell line derived in 1979 from a bone marrow metastasis from a 11 year old Caucasian male with poorly differentiated embryonal neuroblastoma. |
| Clinical Data： | 11 years
Caucasian
male
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| Receptor Expression： | tumor necrosis factor (TNF) type B, expressed tumor necrosis factor (TNF) type A, expressed |
| Genes Expressed： | tumor necrosis factor (TNF) |
| Cellular Products： | tumor necrosis factor (TNF) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice with a latent period of 4 to 6 weeks |
| Comments： | The cells exhibit high MDR1 expression.
Recombinant tumor necrosis factor (TNF) stimulates the proliferation of SK-N-FI cells in both serum free medium and fetal bovine serum supplemented medium, but has no effect in medium without insulin. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,14 D16S539: 11,12 D5S818: 12,13 D7S820: 8,9 THO1: 6,9.3 TPOX: 8,9 vWA: 16,17 |
| Name of Depositor： | C Helson |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1979 |
| References： | Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792 Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824 Goillot E, et al. Tumor necrosis factor as an autocrine growth factor for neuroblastoma. Cancer Res. 52: 3194-3200, 1992. PubMed: 1317260 |