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微信小章| 产品名称： | SK-HEP-1 |
|---|---|
| 商品货号： | TS210908 |
| Organism： | Homo sapiens, human |
| Tissue： | liver / ascites |
| Cell Type： | Endothelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 52 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The cell line is aneuploid human (XX), with chromosome counts in the hypotriploid range. Normal chromosomes N1, N13, N14, N15, and N16 are clearly under-represented with respect to the copy number of other normal chromosomes, while chromosome N7, N12, and N17 tend to be over-represented in some metaphases. Eight markers are identified: del(1)(q21), der(1)t(1;2)(p36;q21), ?tdic(1;12)(p36;q24), ampl.t(4pter--->4q11::HSR::4q12--->4q24::HSR?::4q26--->4q35::2q15--->2pter), 13p+, 13p++, 14p+, t(14q15q), iso(14q), 19q+, del(3)(p14p24), 15p+. |
| Images： |  |
| Clinical Data： | 52 years Caucasian male |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms large cell carcinoma consistent with hepatoma |
| Comments： | The SK-HEP-1 line has been identified as being of endothelial origin. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 8,12 D16S539: 12 D5S818: 10,13 D7S820: 8,11 THO1: 7,9 TPOX: 9 vWA: 14,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1-2 PGM1, 2 PGM3, 1 |
| Name of Depositor： | G Trempe, LJ Old |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1971 |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Heffelfinger SC, et al. SK HEP-1: a human cell line of endothelial origin. In Vitro Cell. Dev. Biol. 28A: 136-148, 1992. PubMed: 1371504 Gucev ZS, et al. Evidence for insulin-like growth factor (IGF)-independent transcriptional regulation of IGF binding protein-3 by growth hormone in SKHEP-1 human hepatocarcinoma cells. Endocrinology 138: 1464-1470, 1997. PubMed: 9075703 Turner BM, Turner VS. Secretion of alpha1-antitrypsin by an established human hepatoma cell line and by human/mouse hybrids. Somatic Cell Genet. 6: 1-14, 1980. PubMed: 6245472 |
| Cross References： | Nucleotide (GenBank) : S40179 pancreatic cholesterol esterase homolog {exon 10} human, SK-Hep-I cells, ATCC HTB 52, Genomic, 84 nt. |